Blood work for athletes
If you train seriously, blood is the only objective window into what is happening under the hood. Recovery, hormones, inflammation and iron status can all be measured. But only if the draw happens at the right moment.
Testosterone and cortisol must be drawn before 10:00
This is the most important point on this page. Testosterone and cortisol follow a daily rhythm. Both peak in the early morning and decline through the day. The reference ranges your laboratory compares you against are built on draws taken before 10:00.
Draw at 15:00 and you measure a trough while comparing it against a morning reference. Your result looks lower than it is, and a follow-up draw at yet another time is not comparable to the first. For someone trying to see whether a change worked, that is the difference between usable and worthless data.
Mario visits in the morning. You do not have to travel across town fasting to reach a collection point in time. You get up, you draw at your own kitchen table, and the timing is right. For a follow-up we book the same window so the two are comparable.
Book a morning slot
Message which window suits you. Hormones get scheduled before 10:00, and for a second measurement Mario keeps the same time so you can lay the two side by side.
Reachable Monday to Saturday, 08:00 to 18:00. An appointment can usually be scheduled within a few days.
What athletes measure
Hormones and recovery
Total and free testosterone, SHBG, LH, oestradiol and morning cortisol. See testosterone.
Load and overreaching
Creatine kinase reflects muscle damage and is high after hard training, so do not draw right after a session. hs-CRP reflects low-grade inflammation. Ferritin can be low in endurance athletes through loss and increased turnover.
Blood count and oxygen transport
Haemoglobin, haematocrit, red cells and ferritin. In endurance sport this speaks to oxygen transport. On testosterone, haematocrit is a value you watch because it can rise.
Liver, kidneys and lipids
With high protein intake, many supplements or compound use, ALT, AST, GGT, creatinine and the lipid profile are the logical checks.
Hormone recovery after anabolic use
Anyone who has stopped wants to know whether their own production returns. You track that with testosterone, LH, FSH and oestradiol, measured at fixed times and repeated over weeks. A single measurement says little here, the series says everything.
This is insight, not treatment. Mario Test does not prescribe and does not supervise cycles. Acting on a result is a conversation with a doctor.
Packages for athletes
More background on the Athletes and Gym page.
Preparation that keeps your result usable
- No hard training in the 48 hours before. It distorts creatine kinase, hs-CRP and liver values.
- Fast if glucose, insulin or triglycerides are included. At least 8 hours.
- Draw at the same time as last time, otherwise you are comparing clocks, not values.
- Same conditions. Comparable sleep, no alcohol the night before.
Frequently asked questions
Why must testosterone be drawn before 10:00?
Because the reference ranges are based on morning draws.
Testosterone follows a daily rhythm and peaks in the early morning. Drawing later gives a lower value that does not match the reference. Mario visits in the morning for this reason.
Can I draw after training?
Better not.
Keep at least 48 hours. Hard training raises creatine kinase, hs-CRP and sometimes liver values for several days.
How often do I need to measure to see anything?
At least twice, 8 to 12 weeks apart.
One measurement is a snapshot. The comparison between two draws at the same time of day shows whether a change worked.
Can you come to my gym?
Yes, the draw can happen at any address.
Home, office or gym. A chair and a table is all that is needed, everything else comes along.
I use testosterone. Which values do I watch?
Haematocrit, haemoglobin, oestradiol, lipids and liver values.
The TRT panels are built around that. This is monitoring and insight, not supervision. For changes to a treatment you see a doctor.
Can you tell me if I am overtrained?
No, overtraining is not established with a blood test.
You do see markers that often accompany it, such as raised creatine kinase, raised hs-CRP and shifting hormone ratios. That is direction, not a conclusion.
Note: a blood test gives you insight and direction. It does not prove or rule out a condition on its own. Results are meant for prevention and for tracking change over time, not as a diagnosis. For symptoms or values outside the reference range, see your GP.